MEK1 ( total ) EIA Kit description

The MEK1 (total) EIA Kit is a solid appearance sandwich agitator affiliated immunosorbent appraisal that provides a fast, acute adjustment to ascertain specific levels of signaling targets in denatured corpuscle extracts. The MEK1 bowl is coated with a specific abrasion monoclonal anti-MEK1 abduction antibiotic on the microwells of the 96-well bright plate. Sample lysate or the accepted included in the kit are incubated in the microwells acceptance MEK1 antigen to be captured in the bowl wells. The bowl is again done to abolish any non-bound ambiguous material. The wells are again incubated with a specific aerial anti-MEK1 antibiotic to ascertain the captured MEK1 on the bowl well. The absolved apprehension antibiotic is done abroad followed by evolution with an HRP-conjugated anti-rabbit antibody.


MEK1 (total) EIA Kit abundant easier and beneath time arresting than Western blotting. After-effects in just 3 hours. Acute to 139 pg/mL. MEK is a bifold specificity kinase able of phosphorylating both tyrosine and threonine residues. The MEK family, which is aswell accepted as MAP kinase kinase, phosphorylates MAP kinases on the conserved T-X-Y motif; phosphorylation of MAPK by MEK after-effects in an access in MAPK activity. MEK is complex in a assorted arrangement of cellular processes such as stress-activated response, apoptosis, cytokine-induced corpuscle proliferation, and DNA recombination during meiosis. The kit aswell includes a accepted that is run as both a absolute ascendancy and to advance a accepted curve. For analysis use only, not for analytic or ameliorative use.

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From: phosphorylates