Application of Phosphodiesterase PDE2A Recombinant Protein

Phosphodiesterase PDE2A Recombinant Protein is to access the appliance of recombinant DNA and recombinant RNA technology of protein. The access can be disconnected into in vitro and in vivo method. Two methods are the appliance of recombinant DNA technology, get affiliated with can be translated into the recombinant gene fragment of ambition protein, again put it into the host beef can accurate the ambition protein announcement of recombinant protein molecules specific to. Including prokaryotic beef such as Escherichia coli, eukaryotic beef such as yeast, insect corpuscle and CHO corpuscle announcement agent of recombinant protein of accepted applications, the recombinant protein assembly can use transgenic beastly breast or plant, the recombinant protein as a artefact of biological medicine, a cogent role in medicine. The use of abiogenetic engineering technology, can accomplish the corpuscle or beastly itself into a "mass assembly biologic factory". By application transgenic beastly mammary announcement of recombinant protein as an example: the adjustment is that the biologic protein gene and breast protein gene apostle and added authoritative apparatus restructuring together, by microinjection adjustment into beastly (mammals, alone lactation) fertilized eggs, and then, the eggs into the mother's body, so that its advance and development transgenic animal. Transgenic beastly entered the lactation period, may be bare for the assembly of protein drugs through the beard of milk, so alleged beastly mammary gland bioreactor or breast bioreactor. At present, scientists accept in the mammary gland bioreactor beasts and goats beastly was apparent in the biologic articles of antithrombin, serum albumin, advance hormone and alpha antitrypsin and added important. At present, the recombinant protein in the biologic industry mainly refers to the announcement of cytokines, acquired agglomeration agency or artificially advised protein molecules.

Phosphodiesterase PDE2A Recombinant Protein adjustment for identification of abrasion lymphocyte hybridoma ability supernatants or specific monoclonal antibiotic affection ablution of monoclonal antibiotic chic and bracket by application bifold antibiotic (can be disconnected into IgG1\\IgG2a\\IgG2b\\IgG3\\IgM\\IgA). Application the abrasion antibiotic were sites of two anti - coated microtiter plate, accumulated with the accession of the ability afloat of abrasion antibodies, antibiotic adjoin abrasion types, subtypes and HRP markers were added to reaction, assuredly TMB substrate arrangement blush and concluded with adulterate sulfuric acid, and again through the agitator mark apparatus barometer absorbance. Judging the detected monoclonal antibiotic chic or subclass. The kit has top resolution, is a reliable apparatus for identification of class, your abrasion monoclonal antibiotic subclasses. Identification of monoclonal antibiotic to antigen, has the actual big aberration with the polyclonal antibody. Altered kit is altered because of the use of monoclonal antibodies, antigen acceptance sites of different, advance to the apprehension aftereffect is difference. Therefore, affiliated questions charge added study. Monoclonal antibiotic was affection ligand important chromatography. The monoclonal antibiotic adsorbed on the solid cast of an apathetic (such as Speharose 2B, 4B, 6B etc.), and able into chromatographic column. When the sample flows through the chromatography column, the break of the antigen can be accumulated with solid-phase mAb specificity, the actual basic does not bind. The chromatographic cavalcade absolutely afterwards elution, alteration the eluent ionic backbone or pH, to the antigen and antibiotic break separation, accession the eluted band-aid can be acquired for the antiseptic antigen. For a bump antigen monoclonal antibiotic is affiliated with chemotherapy or radiation, guided by the monoclonal antibiotic drugs or radiation, agitated to the ambition organ, the absolute killing of ambition cells, accepted as bump targeting therapy. In addition, the radioactive brand is affiliated with the monoclonal antibody, for radioimmunoimaging in patients with analysis of bump implantation, assist.

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